FAT对K562/A02细胞内GSH含量的影响(1)
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[摘要] 目的：探讨FAT对K562/A02细胞内谷胱甘肽(GSH)含量的影响，及其对白血病细胞代谢解毒系统介导的多药耐药(MDR)的逆转效果。方法：采用生化DTNB法测定细胞内谷胱甘肽水平。结果：K562/A02细胞内GSH含量(231.54 μmol/106细胞)高于K562细胞内GSH含量(58.03 μmol/106细胞)，为K562细胞的3.99倍；VRP(10 μmol/L)、FAT(0.01、0.02、0.04、0.08 mg/ml)作用48 h后，K562/A02细胞内GSH含量从231.54 μmol/106细胞分别减少到96.95 μmol/106细胞、212.89 μmol/106细胞、161.00 μmol/106细胞、122.35 μmol/106细胞。结论：细胞内GSH含量增加是K562/A02细胞产生MDR的机制之一；FAT降低K562/A02细胞内GSH的含量是FAT逆转MDR的机制之一。
[关键词] 白血病；多药耐药；FAT；谷胱甘肽
[中图分类号] R-33 [文献标识码] A [文章编号] 1674-4721(2012)01(b)-005-02
Effect of FAT on K562/A02 intracellular GSH concentration
ZHANG Yuxia1, YONG Guoxin1, LIANG Qiong2
1.Department of Technology Application, Hainan Technology and Business College in Hainan Province, Haikou 570203, China; 2.Department of Engineering Technology, Haikou College of Economics in Hainan Province, Haikou 570203, China
[Abstract] Objective: To investigate the impact of FAT on K562/A02 intracellular glutathione (GSH) concentration, and leukemia cell metabolism system of detoxification mediated drug-resistant (MDR) reversal effect. Methods: Intracellular GSH concentration was examined by biochemical DTNB method. Results: Intracellular GSH concentration in K562/A02 cell line (231.54 μmol/106 cells) was higher than that in K562 cell line (58.03 μmol/106 cells), and 3.99-fold greater in K562/A02 cell line compared with in K562 cell line. Intracellular GSH levels in K562/A02 cell line treated with VRP (10 μmol/L), FAT (0.02, 0.04, 0.08 mg/ml) for 48 hours (96.95 μmol/106 cells, 212.89 μmol/106 cells, 161.00 μmol/106 cells,122.35 μmol/106 cells respectively) was decreased than that in K562/A02 cell line without FAT (231.54 μmol/106 cells). The intracellular GSH concentration in K562/A02 cell line was higher than that in K562 cell line. Conclusion: The intracellular GSH content increase in K562/A02 cells to produce MDR mechanism of K562/A02. One of the mechanisms of MDR reversal by FAT is decrease of intracellular GSH levels on K562/A02 ......
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