1延缓造血干/祖细胞衰老中的作用> SIRT6/NF—κB信号轴在人参皂苷Rg1延缓造血干/(1)
[摘要]目的：探讨SIRT6/NF-κB信号轴在人参皂苷Rg1延缓造血干/祖细胞衰老中的作用，为寻找延缓HSC/HPC衰老途径提供理论和实验依据。方法：免疫磁性分选法分离纯化Sca-1+HSC/HPC后分为：正常对照组、衰老组、阳性对照组、Rg1延缓衰老组、Rg1治疗衰老组。衰老相关β-半乳糖苷酶(SA-β-Gal)细胞化学染色、流式细胞术分析细胞周期和造血祖细胞混合集落(CFU-Mix)培养确定Rg1延缓或治疗Sca-1+HSC/HPC衰老生物学作用。荧光定量PCR及Western blotting检测衰老调控分子SIRT6，NF-κB mRNA及蛋白的表达。 结果：与衰老组相比，Rg1延缓及治疗衰老组SA-β-gal染色阳性细胞百分比降低，G1期细胞比例下降，生成CFU-Mix数量增加，SIRT6 mRNA及蛋白表达上调，NF-κB mRNA及蛋白表达下调;Rg1延缓衰老组各指标变化均较Rg1治疗衰老组明显。结论：Rg1可能通过调控SIRT6- NF-κB信号通路发挥其对抗t-BHP诱导的Sca-1+HSC/HPC衰老的作用。
, 百拇医药
[关键词]人参皂苷Rg1;SIRT6;NF-κB;Sca-1+HSC/HPC;衰老
[收稿日期]2014-06-01
[基金项目]国家自然科学基金项目(81202785，81173398)
[通信作者]*周玥，副教授，Tel：(0872)2257140，E-mail：zhouyue_120@sina.comEffect of SIRT6/NF-κB signal axis in delaying hematopoietic stem/
progenitor cell senescence with ginsenoside Rg1
ZHOU Yue1*， TANG Yan-long2， WANG Ya-ping3， WANG Jian-wei3， DING Ji-chao1
, 百拇医药
(1. Department of Histology and Embryology， Dali University， Dali 671000， China;
2. Radiology Department， Affiliated Hospital of Dali University， Dali 671000， China;
3. Laboratory of Stem Cell and Tissue Engineering， Department of Histology and Embryology，Chongqing Medical University， Chongqing 400016， China)
[Abstract]Objective： To investigate the effect of SIRT6/NF-κB signal axis in delaying hematopoietic stem/progenitor cell senescence with ginsenoside Rg1， in order to provide theatrical and experimental basis for looking for methods for delaying HSC senescence. Method： Sca-1+HSC/HPC was isolated by magnetic cell sorting (MACS) and divided into five groups： the normal control group， the aging group， the positive control group， the Rg1 anti-senescence group， and the Rg1-treated group. Senescence-associated β-galactosidase (SA-β-Gal) staining， cell cycle analysis and hemopoietic progenitor cell mix(CFU-Mix) were adopted to determine the effect Rg1 in delaying or treating Sca-1+HSC/HPC senescence biology. The mRNA and protein of senescence regulation molecules SIRT6 and NF-κB were examined by realtime fluorescence quantitative PCR (FQ-PCR) and western blotting. Result： Compared with the senescence group， the Rg1 anti-senescence group and the Rg1-treated group showed lower percentage in SA-β-Gal-stained positive cells， decreased cell proportion in G1 phase， increased number of CFU-Mix， up-regulated in SIRT6 mRNA and protein expression， down-regulation in NF-κB mRNA and protein expression. The Rg1 anti-senescence group showed more evident changes in indexes than the Rg1-treated group. Conclusion： Rg1 may inhibit Sca-1+HSC/HPC senescence induced by t-BHP by regulating SIRT6/NF-κB signal path., 百拇医药(周玥 唐艳隆 王亚平 王建伟 丁继超)
, 百拇医药
[关键词]人参皂苷Rg1;SIRT6;NF-κB;Sca-1+HSC/HPC;衰老
[收稿日期]2014-06-01
[基金项目]国家自然科学基金项目(81202785，81173398)
[通信作者]*周玥，副教授，Tel：(0872)2257140，E-mail：zhouyue_120@sina.comEffect of SIRT6/NF-κB signal axis in delaying hematopoietic stem/
progenitor cell senescence with ginsenoside Rg1
ZHOU Yue1*， TANG Yan-long2， WANG Ya-ping3， WANG Jian-wei3， DING Ji-chao1
, 百拇医药
(1. Department of Histology and Embryology， Dali University， Dali 671000， China;
2. Radiology Department， Affiliated Hospital of Dali University， Dali 671000， China;
3. Laboratory of Stem Cell and Tissue Engineering， Department of Histology and Embryology，Chongqing Medical University， Chongqing 400016， China)
[Abstract]Objective： To investigate the effect of SIRT6/NF-κB signal axis in delaying hematopoietic stem/progenitor cell senescence with ginsenoside Rg1， in order to provide theatrical and experimental basis for looking for methods for delaying HSC senescence. Method： Sca-1+HSC/HPC was isolated by magnetic cell sorting (MACS) and divided into five groups： the normal control group， the aging group， the positive control group， the Rg1 anti-senescence group， and the Rg1-treated group. Senescence-associated β-galactosidase (SA-β-Gal) staining， cell cycle analysis and hemopoietic progenitor cell mix(CFU-Mix) were adopted to determine the effect Rg1 in delaying or treating Sca-1+HSC/HPC senescence biology. The mRNA and protein of senescence regulation molecules SIRT6 and NF-κB were examined by realtime fluorescence quantitative PCR (FQ-PCR) and western blotting. Result： Compared with the senescence group， the Rg1 anti-senescence group and the Rg1-treated group showed lower percentage in SA-β-Gal-stained positive cells， decreased cell proportion in G1 phase， increased number of CFU-Mix， up-regulated in SIRT6 mRNA and protein expression， down-regulation in NF-κB mRNA and protein expression. The Rg1 anti-senescence group showed more evident changes in indexes than the Rg1-treated group. Conclusion： Rg1 may inhibit Sca-1+HSC/HPC senescence induced by t-BHP by regulating SIRT6/NF-κB signal path., 百拇医药(周玥 唐艳隆 王亚平 王建伟 丁继超)