Caco—2细胞研究黄芪中活性成分毛蕊异黄酮的吸收转运(4)
Absorption and transportation of calycosin in Astragali Radix by using
Caco-2 monolayer model
ZHOU Le1， ZHAO Xiao-li1，2，3*， DI Liu-qing1，2，3*
(1.College of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210046， China；
2.Jiangsu Engineering Research Center for Efficient Delivery System of Traditional Chinese Medicine， Nanjing 210046， China；
3. Nanjing Engineering and Technology Research Center of Traditional Chinese Medicine Pellets， Nanjing 210046， China)
[Abstract] Flavonoids are a class of important active ingredients in traditional Chinese medicine， pharmacological activity and in vivo process is the focus of research in recent years. Calycosin is the main active ingredients of flavonoids in Astragali Radix， recent studies indicate that it has many kinds of pharmacological activity， but the absorption and transport characteristics in vivo is unclear. The experiment using Caco-2 cell model， with apigenin as internal standard substance， using the method for the determination of drug concentration by HPLC， were studied at different concentrations and absorption transport characteristics of respectively adding different types of protein inhibitors. Data were analyzed by Q test， the results show that low， middle， high concentration of Papp(BL-AP)/Papp(AP-BL)=1.38<1.5， respectively adding different types of protein inhibitors， compared with the control group of Papp(BL-AP)/Papp(AP-BL)， there were no significant differences. Calycosin absorption may mainly passive transport， also involved in active transport mechanism， the transport may not be affected by the P-protein， MRP2 protein， SGLT protein.
[Key words] Caco-2 model； calycosin； absorption； transportation
doi：10.4268/cjcmm20140932, http://www.100md.com(周乐 赵晓莉 狄留庆)
Caco-2 monolayer model
ZHOU Le1， ZHAO Xiao-li1，2，3*， DI Liu-qing1，2，3*
(1.College of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210046， China；
2.Jiangsu Engineering Research Center for Efficient Delivery System of Traditional Chinese Medicine， Nanjing 210046， China；
3. Nanjing Engineering and Technology Research Center of Traditional Chinese Medicine Pellets， Nanjing 210046， China)
[Abstract] Flavonoids are a class of important active ingredients in traditional Chinese medicine， pharmacological activity and in vivo process is the focus of research in recent years. Calycosin is the main active ingredients of flavonoids in Astragali Radix， recent studies indicate that it has many kinds of pharmacological activity， but the absorption and transport characteristics in vivo is unclear. The experiment using Caco-2 cell model， with apigenin as internal standard substance， using the method for the determination of drug concentration by HPLC， were studied at different concentrations and absorption transport characteristics of respectively adding different types of protein inhibitors. Data were analyzed by Q test， the results show that low， middle， high concentration of Papp(BL-AP)/Papp(AP-BL)=1.38<1.5， respectively adding different types of protein inhibitors， compared with the control group of Papp(BL-AP)/Papp(AP-BL)， there were no significant differences. Calycosin absorption may mainly passive transport， also involved in active transport mechanism， the transport may not be affected by the P-protein， MRP2 protein， SGLT protein.
[Key words] Caco-2 model； calycosin； absorption； transportation
doi：10.4268/cjcmm20140932, http://www.100md.com(周乐 赵晓莉 狄留庆)