HPLC测定蒙药德都红花七味丸中羟基红花黄色素A的含量(2)
本试验用甲醇提取蒙药德都红花七味丸，能使样品中的羟基红花黄色素A得到良好的提取效果。该测定方法简单、结果准确可靠、重复性好，本法可用于德都红花七味丸中羟基红花黄色素A的含量测定。
[参考文献]
[1] 中华人民共和国卫生部.中华人民共和国卫生部药品标准·蒙药分册[S].北京：人民卫生出版社，1998：194.
[2] 中国药典.一部[S].2010： 565.
[3] 梁妍，石任兵，梁吉春.HPLC法测定黄芩总黄酮部位3中成分的含量[J].北京中医药大学学报，2009，32(4)：263.
HPLC determination of hydroxysafflor yellow A in Mongolian medicine Dedu Honghuaqiwei pill
CHEN Chang-lian， HU Si-le
(1. Affiliated Hospital of Inner Mongolia University for the Nationalities，Tongliao 028000， China；
2. Xilingol Mongolian Medicine Research Institute， Xinlingol 026000， China)
[Abstract] Objective： To establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill. Method： The determination was performed by HPLC method on Diamonsil C18 (4.6 mm×250 mm，5 μm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26∶2∶72) as mobile phase. The column temperature was 30 ℃ and the flow rate was 1.0 mL·min-1. Result： The linear rang of hydroxysafflor yellow A was 0.068-0.408 μg and the recovery was 97.66%. Conclusion： The result is accurate with good resolution， and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.
[Key words] Mongolian medicine； Dedu Honghuaqiwei pill； hydroxysafflor yellow A； high performance liquid chromatography
doi：10.4268/cjcmm20122335
[责任编辑 吕冬梅], http://www.100md.com(陈常莲 胡斯乐)
[参考文献]
[1] 中华人民共和国卫生部.中华人民共和国卫生部药品标准·蒙药分册[S].北京：人民卫生出版社，1998：194.
[2] 中国药典.一部[S].2010： 565.
[3] 梁妍，石任兵，梁吉春.HPLC法测定黄芩总黄酮部位3中成分的含量[J].北京中医药大学学报，2009，32(4)：263.
HPLC determination of hydroxysafflor yellow A in Mongolian medicine Dedu Honghuaqiwei pill
CHEN Chang-lian， HU Si-le
(1. Affiliated Hospital of Inner Mongolia University for the Nationalities，Tongliao 028000， China；
2. Xilingol Mongolian Medicine Research Institute， Xinlingol 026000， China)
[Abstract] Objective： To establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill. Method： The determination was performed by HPLC method on Diamonsil C18 (4.6 mm×250 mm，5 μm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26∶2∶72) as mobile phase. The column temperature was 30 ℃ and the flow rate was 1.0 mL·min-1. Result： The linear rang of hydroxysafflor yellow A was 0.068-0.408 μg and the recovery was 97.66%. Conclusion： The result is accurate with good resolution， and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.
[Key words] Mongolian medicine； Dedu Honghuaqiwei pill； hydroxysafflor yellow A； high performance liquid chromatography
doi：10.4268/cjcmm20122335
[责任编辑 吕冬梅], http://www.100md.com(陈常莲 胡斯乐)